Bscs are designed to be connected to the laboratory exhaust system in one of two ways.
Biosafety cabinet biosafety level 3 laboratory design.
Bscs can also be connected to the laboratory exhaust system.
Supply air must be provided in such a manner that prevents positive pressurization of the cabinet.
Preferably this is a class ii biosafety cabinet or equivalent containment device for work with agents and an autoclave or other suitable method for decontamination within the lab.
The biosafety officer in collaboration with the institutional biosafety committee must approve the location and design of any bsl 3 facility and has final authority to authorize the commencement of bsl 3 work the biosafety level 3 facility design and operational procedures must be documented.
Basic laboratory design for biosafety levels 1 and 2.
Laboratories covers all design requirements for biosafety level 1 laboratory work areas.
Please see biosafety level 3 guidelines for bsl3 laboratories.
This section focuses primarily on the biosafety considerations for a biosafety level 2 laboratory.
Hepa filtered exhaust air from a class ii bsc can be safely recirculation back into the laboratory environment if the cabinet is tested and certified at least annually and operated according to manufacturer s recommendations.
A readily available eyewash station is needed.
Laboratory containment for human pathogens.
Class ii a2 biosafety cabinets 3 ft.
The assignment of a biosafety level to a particular work process is made through a protocol driven risk assessment.
Table 1 describes the risk groups table 2 relates them to the laboratory designations and table 3 summarizes the requirements at the four biosafety levels.
Facilities as basic biosafety level 1 basic biosafety level 2 containment biosafety level 3 and maximum containment biosafety level 4.
The facility must be tested for verification that the design and operational parameters have been.
Selfclosing lockable doors and biohazard warning signs are also required at all access points.
For more information on other aspects of the biosafety levels please see the rest of the biosafety section for a general overview.
Work at bsl 3 requires enhanced facility design operational controls and special practices which will be outlined in this section.
Basic laboratory design for biosafety level 3.
Biosafety level 1 biosafety level 4 facilities.
Date laboratory location responsible individual queries are based on the biosafety level 3 section of the biosafety in microbiological and biomedical laboratories 5th edition 2007 circle the response that best describes the laboratory in which work will be performed.