Bioreactors For Animal Cell Culture

In general a conventional submerged type of bioreactor oxygenator with agitation system has been used in cell cultivations.

Bioreactors for animal cell culture.

One bacterial spore or vegetative bacterial cell is all it takes to overgrow an animal cell culture. The biological systems involved include enzymes microorganisms animal cells plant cells and tissues. Bioreactors play a key role in the field of biologics where they are used for the production of recombinant therapeutic proteins by large scale cultivation of animal cells. Critical contamination points for a bioreactor are the transitions between sterile spaces such as the stirrer ports and gas lines.

Home environment animal cell culture prev contents next bioreactors for suspension culture. This process can either be aerobic or anaerobic these bioreactors are commonly cylindrical ranging in size from litres. In one case a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. Requirements for a bioreactor for animal cell culture 1 well controlled environment t ph do nutrients and wastes 2 supply of nutrients 3 gentle mixing avoid shear damage to cells 4 gentle aeration add oxygen slowly to the culture medium but avoid the formation of large bubbles which can damage cells on contact.

There are several types of bioreactors including stirred tank airlift hollow fiber and rotary cell culture system rccs d. A bioreactor refers to any manufactured device or system that supports a biologically active environment. Bioreactors for suspension culture belong to the group of dynamic systems compare sect. 4 1 1 and can be either mechanically driven stirred tank bioreactor or pneumatically driven bubble column air lift bioreactor.

There are three major types of homogeneous oxygenator systems used for. Microcarriers with adherent cells which were in principle an adaptation of homogeneous culture systems used for. However despite this sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen especially on a large scale. This article examines the underlying mechanisms involved in bubble associated cell damage and the methods available for controlling such damage.

Animal cell culture bioreactors initially employed stirred tanks containing. Cell cultures are particularly susceptible to biological contamination. The bioreactor is a place where an optimum external. The gas sparging of culture broth causes damage to suspended animal cells.

Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture.